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The peak fractions were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (Cytiva) pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 1 mM TCEP)
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Size:50 mg
Valentine, S
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