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Fifteen min after the application of short-circuit conditions, the cells were incubated with glucose (10 mM, mucosal, diluted in aqua destillata) and mannitol (10 mM, serosal, diluted in aqua destillata) for 20 min, followed by a 10 min incubation period with carbachol (10 μM, serosal, Sigma-Aldrich, diluted in aqua destillata) and a final incubation with forskolin (10 μM, serosal, Sigma-Aldrich, diluted in Dimethyl sulfoxide) for another 10 min

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